Journal: International Journal of Molecular Sciences

Article Title: Effects of Palm Oil Tocotrienol-Rich Fraction (TRF) and Carotenes in Ovalbumin (OVA)-Challenged Asthmatic Brown Norway Rats

doi: 10.3390/ijms20071764

Figure Lengend Snippet: Effects of palm oil TRF, carotenes, and dexamethasone on pro-inflammatory cytokines, ( a ) IL-1β, ( b ) IL-6, and ( c ) TNF-α, in the plasma of Brown Norway rats challenged with OVA. Data are presented as the means ± SD, n = 10 rats per group. a P < 0.05 versus the control group. b P < 0.05 versus the OVA-challenged group.

Article Snippet: Standard enzyme linked immunosorbent assay (ELISA) kits used for the determination of rat IL-β, IL-6, IL-4, IL-13, TNF-α (Novateinbio, Woburn, Massachusetts, USA), CRP (eBioscience, Affymetrix, Santa Clara, California, USA), IgE, LTB-4, and LTB-5 (MyBioSource, San Diego, California, USA) were utilized.

Techniques:

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: The levels of serum IL-31 were assessed in patients with asthma (n = 44) and controls (n = 22) as measured by ELISA. ( A ) Serum IL-31 was increased in patients with asthma compared with the controls; ( B ) When patients were stratified according to the status of atopy, the serum IL-31 levels in patients with allergic asthma (n = 23) were higher than those in patients with nonallergic asthma (n = 21) and controls; ( C ) Serum IL-31 was increased in severe asthma patients (n = 22) compared with mild asthma patients (n = 22) and controls; ( D ) Receiver operating characteristic (ROC) curve for distinguishing patients with asthma from the controls. The area under the ROC curve was 0.791 (95% CI: 0.66 to 0.86). Data are presented as median with interquartile range. *p < 0.05, ***p < 0.001.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: ( A ) BALF samples were measured by ELISA and the BALF IL-31 levels in patients with asthma (n = 9) were significantly increased compared with the controls (n = 5); ( B ) Spearman’s rank correlation analysis showed the serum IL-31 levels correlated positively with BALF IL-31 levels ( r = 0.63; p = 0.016). Data are presented as median with interquartile range. **p < 0.01.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: The classical Th2 cytokines such as IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were further measured by ELISA. The levels of serum IL-5, IL-13 and TSLP were significantly higher in patients with asthma (n = 44) compared with control subjects (n = 22) ( A–C ). Spearman’s rank correlation analysis showed a significant correlation between the serum IL-31 levels and the serum IL-5 levels ( r = 0.34, p = 0.024), the serum IL-13 levels ( r = 0.43, p = 0.003), the serum TSLP levels ( r = 0.42, p = 0.004) ( D–F ). Data are presented as median with interquartile range. **p < 0.01, ***p < 0.001.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular Medicine Reports

Article Title: Curcumin inhibits advanced glycation end product-induced oxidative stress and inflammatory responses in endothelial cell damage via trapping methylglyoxal

doi: 10.3892/mmr.2015.4725

Figure Lengend Snippet: Regulation of Cur-MGO adducts on inflammatory cytokines (A and B) ICAM-1 and (C and D) TGF-β1 expression in HUVECs by immunocytochemistry. a, Control; b, HSA; c, MGO; d, MGO + HSA; e-g, MGO + HSA in the presence of 10 −7 , 10 −6 and 10 −5 M Cur, respectively; h, MGO + HSA in the presence of 10 −6 M AG. This experiment was performed three times. The data are presented as the mean ± standard deviation (n=3). ** P<0.01, vs. HSA; # P<0.05 and ## P<0.01, vs. MGO and HAS+MGO. Cur, curcumin; MGO, methylgloxal; ICAM-1, intercellular adhesion molecule-1; TGF-β1, transforming growth factor-β1; HUVECs, human umbilical vein endothelial cells; HSA, human serum albumin; AG, aminoguanidine hydrochloride.

Article Snippet: Cells were then incubated with biotin-conjugated secondary antibodies ICAM1 and TGF-β1 for 120 min at room temperature, and the staining was visualized using 3,3-diaminobenzidine (Nanjing KeyGen Biotech Co., Ltd.).

Techniques: Expressing, Immunocytochemistry, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Curcumin inhibits advanced glycation end product-induced oxidative stress and inflammatory responses in endothelial cell damage via trapping methylglyoxal

doi: 10.3892/mmr.2015.4725

Figure Lengend Snippet: Effect of Cur-MGO adducts on the expression of the inflammatory cytokines ICAM-1 and TGF-β1 in HUVECs by western blot analysis. (A) The bands of ICAM-1 and TGF-β1; (B) the relative expression levels of ICAM-1 and TGF-β1. a, HSA; b, MGO; c, MGO + HSA; d, MGO + HSA + Cur (10 −6 M); e, MGO + HSA + Cur (10 −5 M); f, MGO + HSA + AG (10 −6 M). Data are presented as the mean ± standard deviation (n=3). ** P<0.01, vs. HSA; # P<0.05, ## P<0.01, vs. MGO or MGO + HSA. Cur, curcumin; MGO, methylgloxal; ICAM-1, intercellular adhesion molecule-1; TGF-β1, transforming growth factor-β1; HUVECs, human umbilical vein endothelial cells; HSA, human serum albumin; AG, aminoguanidine hydrochloride.

Article Snippet: Cells were then incubated with biotin-conjugated secondary antibodies ICAM1 and TGF-β1 for 120 min at room temperature, and the staining was visualized using 3,3-diaminobenzidine (Nanjing KeyGen Biotech Co., Ltd.).

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Cell Journal (Yakhteh)

Article Title: Melatonin Attenuates Upregulation of Duox1, and Duox2 and Protects against Lung Injury following Chest Irradiation in Rats

doi: 10.22074/cellj.2019.6207

Figure Lengend Snippet: Results of changes in the levels of IL-4 and IL-13 following irradiation with gamma rays and treatment with melatonin (MLT). A. IL-4 and B. IL- 13. a; Significant compared to control and b; Significant compared to radiation (Rad), ANOVA Tukey’s HSD post hoc, P<0.05.

Article Snippet: The levels of IL-4 and IL-13 cytokines were detected by the IL-4 and IL-13 ELISA kits (Zellbio, Germany) based on the manufacturer’s instructions.

Techniques: Irradiation